Sestambi QC

Sestamibi (Cardiolite, Miraluma) Quality Control


Procedure A

Materials needed:

Aluminum oxide TLC plate
95% Ethanol
Scissors
Pencil/Pen
Counting tubes/vials
MCA or SCA or Scaler with scintillation well

Procedure:

1. See General QC Procedure.

2. “Wet” plate with 1 drop ethanol. Spot sample on top of ethanol spot.

3. Develop immediately in ethanol, allowing the solvent to migrate to the ink spot/line at top of the plate.

4. Remove plate from solvent.

5. Cut plate at cut line above origin.

6. Count each section of the plate.

Counts:

Ethanol:
Top: Tagged radiopharmaceutical
Bottom: Free Tc-99m, hydrolyzed reduced Tc-99m

% tagged Tc-99m = (top counts)/(bottom counts + top counts) x 100

sestamib chart