Tetrofosmin QC

Tetrofosmin (Myoview) Quality Control Procedure


Procedure A

Materials needed:

ITLC SG 10 cm x 1 cm strip
35:65 acetone:dichloromethane
Scissors
Pencil/Pen
Counting tubes/vials
MCA or SCA or Scaler with scintillation well

Procedure:

1. See General QC Procedure. Spot the sample 1.5 cm from the end of strip.

2. Allow solvent to rise to 9 cm from bottom of the strip.

3. Remove strip from solvent.

5. Make two cuts: first cut at 3 cm and second cut at 7.5 cm.

6. Count each 3 parts (origin/top, middle, solvent front/bottom)

Counts:

Top: Free Tc-99m
Middle: Tagged radiopharmaceutical
Bottom: hydrolyzed reduced Tc-99m

% tagged Tc-99m = (middle counts)/(counts from all three sections) x 100

 


Procedure B

Materials needed:

Whatman 31 strip
Ethyl Acetate
Scissors
Pencil/Pen
Counting tubes/vials
MCA or SCA or Scaler with scintillation well

Procedure:

1. See General QC Procedure. Spot the sample 1 cm from the end of strip.

2. To develop the strip, place it in ethyl acetate.

3. Allow the solvent to migrate to the ink spot/line at top of the strip.

4. Remove strip from solvent.

5. Cut strip in half.

6. Count each section of the strip.

Counts:

Ethyl Acetate:
Top: Tagged radiopharmaceutical
Bottom: Free Tc-99m, hydrolyzed reduced Tc-99m

% tagged Tc-99m = (top counts)/(bottom counts + top counts) x 100

USP Minimum Acceptable Purities:

Myoview 90%