Procedure III

Procedure III

For the following Drugs:

DTPA
HDP
MDP
PYP
Glucoheptonate

Materials needed:

*ITLC-Silica Gel Strip
*Whatman 3MM Strip
*0.9% Saline
*Acetone
Scissors
Pencil/Pen
Counting tubes/vials
MCA or SCA or Scaler with scintillation well

*Alternative media and solvents may be suitable. Use of other solvents and medium must be validated before routine use.

Procedure:

1. SeeĀ General QC Procedure. Spot the sample 1 cm from the end of each strip.

2. To develop the strips, place the Whatman 3MM strip in acetone and the ITLC-SG strip in 0.9% Saline.

3. Allow the solvent to migrate to the ink spot/line at top of the strips.

4. Remove strips from solvents.

5. Cut each strip at cut line above origin.

6. Count each section of both strips, keeping track of which section belongs to which solvent.

Counts:

Strips:

Acetone: Top: Free Tc-99m

Bottom: Tagged drug and hydrolyzed-reduced Tc-99m

Saline: Top: Free Tc-99m and tagged drug

Bottom: Hydrolyzed -reduced Tc-99m

Acetone: % free Tc-99m = (top counts)/ (bottom counts + top counts) X 100

Saline: % HR Tc-99m = (bottom counts)/ (bottom counts + top counts) X 100

% tagged radiopharmaceutical = 100- (% free Tc-99m + % HR Tc-99m)

Procedure 3 graph

USP Minimum Acceptable Purities:

DTPA 90%
HDP 90%
MDP 90%
PYP 90%
Glucoheptonate 90%