Procedure II QC

Procedure II

For the following Drugs:

MAA
DMSA
Sulfur Colloid
Albumin Colloid

Materials needed:

*ITLC-Silica Gel Strip
*0.9% Saline
Scissors
Pencil/Pen
Counting tubes/vials
MCA or SCA or Scaler with scintillation well

*Alternative media and solvents may be suitable. Use of other solvents and medium must be validated before routine use.

Procedure:

1. SeeĀ General QC Procedure. Spot the sample 1 cm from the end of strip.

2. To develop the strip, place it in saline.

3. Allow the solvent to migrate to the ink spot/line at top of the strip.

4. Remove strip from solvent.

5. Cut strip at cut line above origin.

6. Count each section of the strip.

Counts:

Top: contains free Tc-99m sodium pertechnetate

Bottom: contains the tagged drug

% free Tc-99m = (top counts)/(bottom counts + top counts) X 100

% tagged radiopharmaceutical = 100- % free Tc-99m

USP Minimum Acceptable Purities:

MAA 90%
DMSA 90%
Sulfur Colloid 92%