Tetrofosmin (Myoview) Quality Control Procedure
Materials needed:
ITLC SG 10 cm x 1 cm strip
35:65 acetone:dichloromethane
Scissors
Pencil/Pen
Counting tubes/vials
MCA or SCA or Scaler with scintillation well
Procedure:
1. See General QC Procedure. Spot the sample 1.5 cm from the end of strip.
2. Allow solvent to rise to 9 cm from bottom of the strip.
3. Remove strip from solvent.
5. Make two cuts: first cut at 3 cm and second cut at 7.5 cm.
6. Count each 3 parts (origin/top, middle, solvent front/bottom)
Counts:
Top: Free Tc-99m
Middle: Tagged radiopharmaceutical
Bottom: hydrolyzed reduced Tc-99m
% tagged Tc-99m = (middle counts)/(counts from all three sections) x 100
Materials needed:
Whatman 31 strip
Ethyl Acetate
Scissors
Pencil/Pen
Counting tubes/vials
MCA or SCA or Scaler with scintillation well
Procedure:
1. See General QC Procedure. Spot the sample 1 cm from the end of strip.
2. To develop the strip, place it in ethyl acetate.
3. Allow the solvent to migrate to the ink spot/line at top of the strip.
4. Remove strip from solvent.
5. Cut strip in half.
6. Count each section of the strip.
Counts:
Ethyl Acetate:
Top: Tagged radiopharmaceutical
Bottom: Free Tc-99m, hydrolyzed reduced Tc-99m
% tagged Tc-99m = (top counts)/(bottom counts + top counts) x 100
USP Minimum Acceptable Purities:
Myoview 90%